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Image Search Results
Journal: Addiction Biology
Article Title: AMPA Receptor Within the Prelimbic Cortex Regulates Propofol‐Induced Locomotor Sensitization
doi: 10.1111/adb.70078
Figure Lengend Snippet: The expressions of D1R (B), p‐ERK (C) and p‐CREB (D) were statistically significantly decreased by NBQX pretreatment at the dose of 1.0 μg/0.3 μL/site (D1R, p < 0.001, n = 6; p‐ERK, p < 0.001, n = 6; p‐CREB, p = 0.003, n = 6). The expressions of t‐ERK (E, p = 0.976, n = 6) and t‐CREB (F, p = 0.982, n = 6) remained unchanged. One‐way ANOVA was applied for the normally distributed data, with Dunnett's post hoc test for multiple comparisons; otherwise, the data were analysed by Kruskal–Wallis test with the Dunn's post hoc analysis for multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Then, the proteins were transferred to polyvinylidene fluoride (PVDF) membranes (NCM Biotech, Suzhou, China), and treated with NcmBlot Blocking Buffer (NCM Biotech, Suzhou, China) at room temperature for 20 min. After that, the bands were incubated overnight at 4 °C with primary antibodies (NR1, p‐NR1 (Ser896), NR2B, p‐NR2B (Tyr1472), p‐ERK and p‐CREB, rabbit, 1:1000, Cell Signalling Technology, Danvers, MA, USA; GluA1, GluA2, D1R, ERK and
Techniques: